DDDDK-Tag(binds to flag sequnence) rabbit pAb
简要描述：DDDDK-Tag(binds to flag sequnence) rabbit pAb宿主: Polyclonal, Rabbit,IgG反应性: Species independent应用: WB;ELISA;IP;IF
产品型号： YP-Ab-17192
所属分类：标签内参抗体
更新时间：2024-10-31
详细说明：
DDDDK-Tag(binds to flag sequnence) rabbit pAb
抗体，也叫免疫球蛋白（Ig），是一种能特异性结合抗原的糖蛋白，而抗原是在易感染动物体内引发抗体产生的物质。在体内，抗体是由于外源性分子的侵袭而产生的。抗体以一个或者多个Y字形单体存在，每个Y字形单体由4条多肽链组成，包含两条相同的重链和两条相同的轻链。轻链和重链是根据它们的分子量大小来命名的。Y字形结构的顶端是可变区，为抗原结合部位。任何一个抗体的轻链都可以分为κ或λ型（基于小分子多肽结构上的差异），每一个抗体的重链则决定了它的类或型。
产品详情
FLAG-tag Polyclonal Antibody detects FLAG-tagged recombinant proteins or FLAG-tagged proteins overexpressed in cells.
免疫原详细信息
DDDDK synthetic peptide conjugated to KLH.
相关试剂
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.05% sodium azide.
背景
The DYKDDDDK (FLAG) peptide has been used extensively as a general tag in expression vectors. This peptide can be expressed and detected with the protein of interest as an amino-terminal or carboxy-terminal fusion. N-terminal FLAG vectors provide an Ek cleavage site for removal of the fusion tag. The FLAG peptide is likely to be located on the surface of a fusion protein because of its hydrophilic nature. As a result, the FLAG peptide is more likely to be accessible to antibodies. A FLAG-tag can be used in many different assays that require recognition by an antibody, such as western blotting, immunocytochemistry, immunoprecipitation, flow cytometry, protein purification, and in the study of protein-protein interactions, cell ultrastructure, and protein localization and so on.
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备注：图片仅供参考。仅供科研，不可用于临床。
